Evaluating the Wound Healing Activity of Tribulus terrestris L. leaf powder Ointment on Splinted excisional wounds in Balb/C mice
- 03 Nov 2024
- Published Resarch - Pharmacy
| Researchers | Racha Daiea, Jamal Abdul Naser Darwicha, Lina Saker and Aziza Youssef |
| Published in | Research Journal of Pharmacy and Technology, volume 17, issue 7, July 2024. |
| Abstract | Background: Recent advancements in wound healing focus on botanically based medications with expedite the process with increased safety and fewer side effects. While Tribulus terrestris (T.T) L. has been acknowledged for its’ wound healing potential in vivo studies, particularly in murine models, faces challenges due to the role of the muscle layer Panniculus carnosus, leading to false positive results in clinical trials. Objective: This study aims to demonstrate the wound healing activity of powdered T.T leaves by employing a novel technique: splinted full-thickness excisional wounds in mice. Methods: Leaves of T.T were collected, grinded, sieved, and added to a hydrophilic ointment. The experiment was conducted on 50 Balb/C male mice, divided equally to 5 groups: Group I treated with 10% T.T, Group II treated with 15% T.T, Group III treated with MEBO, Group IV treated with ointment excipients, and Group Vwasn’t treated. Using isoflurane gas 5% in propylene glycol as anaesthesia, two excisional wounds were inflicted on the dorsal area on each mouse. Each group was treated with the appropriate treatment once daily and the wound areas were measured every two days for 14 days. Digimizer software was used formeasuring the wound areas then we calculated the wound healing percentages. SPSS software was used to analyse the wound healing percentages data, which they were demonstrated as mean±SD. The performed tests: One-way ANOVA test, Tukey’s test, Pearson’s correlation coefficient test, Dendrogram tree and proximity matrix. Results: A significant difference was obtained when comparing the groups treated with 10% and 15% powdered leaf T.T ointments with the negative control and positive control (MEBO) at P-value=0.000. Group II demonstrated the highest healing rate, followed by Group III then group I then group V, finally group IV. The healing increased with increasing the concentration of the powdered leaf, which was demonstrated by Group II in the first 7 days of the experiment. Pearson’s test proved there was a correlation between time and the healing rate. Dendrogram tree and proximity tree displayed a similarity among Groups I, II, and III, and between groups IV and V. T.T leaves proved their wound healing activity through the synergistic action of the present plentiful phytochemicals in leaves: phenolic acids, flavonoids, tannins, and ß-sitosterol. Conclusion: T.T leaves showed a good healing action in vivo and it was improved by increasing the powder’s concentration, therefore, it decreased the healing period. Key words: Tribulus terrestris, powdered leaves, excisional wounds, splinted wounds, wound healing. |
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